Measurement
Part:BBa_K337032:Design
Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R Group: iGEM10_Heidelberg (2010-10-22)
pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv40/CMV/Kozag_hRluc_BGH )
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
Illegal BamHI site found at 3421
Illegal XhoI site found at 3163 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1547
Illegal NgoMIV site found at 2891
Illegal NgoMIV site found at 2912
Illegal NgoMIV site found at 3184
Illegal AgeI site found at 2615 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4600
Illegal SapI.rc site found at 2797
Design Notes
easy mesurment of microRNA binding site quality
Source
BGH: pcDNA5/FRT/To